CDK1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P06493 |
| Gene Id | 983 |
| Gene Symbols | CDK1 |
| Protein Name / Synonyms | CDK1, CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
27kDa/34kDa
Observed MW
34kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human CDC2 (NP_001777.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CDK1 antibody (144-012414) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CDK1 antibody (144-012414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CDK1 antibody (144-012414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CDK1 antibody (144-012414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDK1 antibody (144-012414) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.