Anti-CDK10 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q15131
Gene Id	8558
Gene Symbols	CDK10
Protein Name / Synonyms	CDK10, PISSLRE, cyclin-dependent kinase 10
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

17kDa/21kDa/30kDa/31kDa/32kDa/41kDa

Observed MW

43kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human CDK10 (NP_001092003.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CDK10 antibody (144-02690) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CDK10 antibody (144-02690)

Immunohistochemistry of paraffin-embedded mouse brain using CDK10 antibody (144-02690) at dilution of 1:100 (40x lens).
Immunohistochemistry - CDK10 antibody (144-02690)

Immunofluorescence analysis of U2OS cells using CDK10 antibody (144-02690) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - CDK10 antibody (144-02690)

Expiration:

12 months from the date of shipment when stored properly.

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