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Anti-CDK4 Antibody
Catalog #: 144-00366
CDK4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P11802 |
| Gene Id | 1019 |
| Gene Symbols | CDK4 |
| Protein Name / Synonyms | CDK4, CMM3, PSK-J3, cyclin-dependent kinase 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IHC 1:50 - 1:200
IF 1:20 - 1:100
Format
Calculated MW
20kDa/33kDa
Observed MW
34kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CDK4 (NP_000066.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CDK4 antibody (144-00366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot analysis of extracts of various cell lines,using CDK4 antibody (144-00366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat lung using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDK4 Antibody (144-00366) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.