Anti-CDK8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P49336
Gene Id	1024
Gene Symbols	CDK8
Protein Name / Synonyms	CDK8, K35, cyclin-dependent kinase 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:100

Format

Calculated MW

53kDa

Observed MW

56kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of Jurkat cells, using CDK8 antibody (144-05548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] CDK8 Polyclonal Antibody (144-05548)

Western blot analysis of extracts from normal (control) and CDK8 knockout (KO) HeLa cells, using CDK8 antibody (144-05548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] CDK8 Polyclonal Antibody (144-05548)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK8 antibody (144-05548). Western blot was performed from the immunoprecipitate using CDK8 antibody (144-05548) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] CDK8 Polyclonal Antibody (144-05548)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products