Anti-CDK8 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P49336
Gene Id	1024
Gene Symbols	CDK8
Protein Name / Synonyms	CDK8 (Center)
Clonality	Polyclonal
Preparation	This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human CDK8.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, FC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Specificity

Predicted Reactivity

Mouse, Zebrafish, Xenopus

Other Names

CDK8; Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35

NCBI Accession #

NP_001251.1

Other Accession #

Q66KH9; Q8R3L8; Q8JH47; NP_001251

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

53284

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	Western blot analysis of CDK8 (arrow) using rabbit polyclonal CDK8 Antibody (Center) (Cat# 102-11341). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK8 gene.
	CDK8 Antibody (Center) (Cat# 102-11341) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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