RayBiotech

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ISO 13485:2016 cGMP

Anti-CEACAM3 Antibody

Catalog #: 144-02589

CEACAM3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P40198
Gene Id
1084
Gene Symbols
CEACAM3
Protein Name / Synonyms
CEACAM3, CD66D, CEA, CGM1, W264, W282, carcinoembryonic antigen-related cell adhesion molecule 3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
19kDa/22kDa/27kDa
Observed MW
38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human CEACAM3 (NP_001806.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CEACAM3 antibody (144-02589) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - CEACAM3 Polyclonal Antibody (144-02589)

Expiration:
12 months from the date of shipment when stored properly.