Anti-CES2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O00748
Gene Id	8824
Gene Symbols	CES2
Protein Name / Synonyms	CES2, CE-2, CES2A1, PCE-2, iCE, cocaine esterase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

59kDa/61kDa

Observed MW

62kDa

Immunogen

A synthetic peptide of human CES2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CES2 antibody (144-01514) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CES2 antibody (144-01514)

Immunohistochemistry of paraffin-embedded human colon using CES2 antibody (144-01514) at dilution of 1:100 (40x lens).
Immunohistochemistry - CES2 antibody (144-01514)

Immunofluorescence analysis of MCF-7 cells using CES2 antibody (144-01514). Blue: DAPI for nuclear staining.
Immunofluorescence - CES2 antibody (144-01514)

Expiration:

12 months from the date of shipment when stored properly.

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