RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-CETN1 Antibody

Catalog #: 144-03784

CETN1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q12798
Gene Id
1068
Gene Symbols
CETN1
Protein Name / Synonyms
CETN1, CEN1, CETN, centrin-1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
19kDa
Observed MW
20kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN1 (NP_004057.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CETN1 antibody (144-03784) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - CETN1 antibody (144-03784)

Immunohistochemistry of paraffin-embedded human liver injury using CETN1 antibody (144-03784) at dilution of 1:100 (40x lens).
Immunohistochemistry - CETN1 antibody (144-03784)

Expiration:
12 months from the date of shipment when stored properly.