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Anti-CETN2 Antibody
Catalog #: 144-05397
CETN2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P41208 |
| Gene Id | 1069 |
| Gene Symbols | CETN2 |
| Protein Name / Synonyms | CETN2, CALT, CEN2, centrin-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
19kDa
Observed MW
21kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CETN2 Antibody (144-05397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunofluorescence analysis of A549 cells using CETN2 antibody (144-05397).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using CETN2 antibody (144-05397). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Expiration:
12 months from the date of shipment when stored properly.