Anti-CFL1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P23528
Gene Id	1072
Gene Symbols	CFL1
Protein Name / Synonyms	CFL1 (N-term)
Clonality	Polyclonal
Preparation	This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human CFL1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

Specificity

Predicted Reactivity

Rat, Bovine, Pig, Monkey

Other Names

CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; Cofilin, non-muscle isoform

NCBI Accession #

NP_005498.1

Other Accession #

P45592; P10668; P18760; Q4R5C0; Q5E9F7

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

18502

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of CFL1 Antibody (N-term) (Cat# 102-15217) in MCF-7,Hela cell line and mouse brain tissue lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab.(2ug/ml)
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CFL1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of hela cells using CFL1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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