Anti-CHAT Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P28329
Gene Id	1103
Gene Symbols	CHAT
Protein Name / Synonyms	CHAT, CHOACTASE, CMS1A, CMS1A2, CMS6, choline O-acetyltransferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

70kDa/74kDa/82kDa

Observed MW

68kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHAT antibody (144-02495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CHAT antibody (144-02495)

Immunohistochemistry of paraffin-embedded rat brain using CHAT antibody (144-02495) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHAT antibody (144-02495)

Expiration:

12 months from the date of shipment when stored properly.

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