Anti-CHD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O14646
Gene Id	1105
Gene Symbols	CHD1
Protein Name / Synonyms	CHD1, chromodomain-helicase-DNA-binding protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200

Format

Calculated MW

196kDa

Observed MW

197kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHD1 Antibody (144-07883) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] CHD1 Polyclonal Antibody (144-07883)

Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (144-07883) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] CHD1 Polyclonal Antibody (144-07883)

Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (144-07883) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (144-07883)

Immunohistochemistry of paraffin-embedded human liver injury using CHD1 antibody (144-07883) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (144-07883)

Immunohistochemistry of paraffin-embedded human lung cancer using CHD1 antibody (144-07883) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (144-07883)

Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (144-07883) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (144-07883)

Expiration:

12 months from the date of shipment when stored properly.

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