CHEK1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14757 |
| Gene Id | 1111 |
| Gene Symbols | CHEK1 |
| Protein Name / Synonyms | CHEK1, CHK1, checkpoint kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | DNA Damage |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
43kDa/50kDa/54kDa
Observed MW
54kDa
Immunogen
A synthetic peptide of human CHEK1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CHEK1 antibody (144-07653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney cancer using CHEK1 antibody (144-07653) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using CHEK1 antibody (144-07653) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CHEK1 antibody (144-07653) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CHEK1 antibody (144-07653) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.