Anti-CHFR (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96EP1
Gene Id	55743
Gene Symbols	CHFR
Protein Name / Synonyms	CHFR (C-term)
Clonality	Polyclonal
Preparation	This CHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-504 amino acids from the C-terminal region of human CHFR.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Specificity

Predicted Reactivity

Mouse

Other Names

CHFR; RNF196; E3 ubiquitin-protein ligase CHFR; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196

NCBI Accession #

NP_001154816.1;NP_001154817.1;NP_001154818.1;NP_001154819.1;NP_060693.2

Other Accession #

Q810L3; NP_001154818.1; NP_001154817.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

73386

Recommended Dilutions

WB: 1:1000

CHFR Antibody (C-term) (Cat# 102-12486) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHFR antibody detected the CHFR protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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