Anti-CHIT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q13231
Gene Id	1118
Gene Symbols	CHIT1
Protein Name / Synonyms	CHIT1, CHI3, CHIT, CHITD, chitinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

43kDa/48kDa/49kDa/51kDa

Observed MW

52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 217-466 of human CHIT1 (NP_003456.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHIT1 antibody (144-02015) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CHIT1 antibody (144-02015)

Immunohistochemistry of paraffin-embedded human normal stomach using CHIT1 antibody (144-02015) at dilution of 1:200 (40x lens).
Immunohistochemistry - CHIT1 antibody (144-02015)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products