RayBiotech

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ISO 13485:2016 cGMP

Anti-CHMP1A Antibody

Catalog #: 144-11621

CHMP1A Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9HD42
Gene Id
5119
Gene Symbols
CHMP1A
Protein Name / Synonyms
CHMP1A, CHMP1, PCH8, PCOLN3, PRSM1, VPS46-1, VPS46A, charged multivesicular body protein 1a
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaAutophagy
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
7kDa/21kDa
Observed MW
35kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human CHMP1A (NP_002759.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHMP1A antibody (144-011621) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - CHMP1A antibody (144-011621)

Expiration:
12 months from the date of shipment when stored properly.