CHN1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P15882 |
| Gene Id | 1123 |
| Gene Symbols | CHN1 |
| Protein Name / Synonyms | CHN1, ARHGAP2, CHN, DURS2, NC, RHOGAP2, chimerin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes GTPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. Mutations in this gene are associated with Duane's retraction syndrome 2 (DURS2). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
38kDa/50kDa/53kDa
Observed MW
53kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CHN1 (NP_001813.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CHN1 antibody (144-07546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using CHN1 antibody (144-07546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CHN1 antibody (144-07546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CHN1 antibody (144-07546) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.