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Anti-CHRFAM7A Antibody
Catalog #: 144-07844
CHRFAM7A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q494W8 |
| Gene Id | 89832 |
| Gene Symbols | CHRFAM7A |
| Protein Name / Synonyms | CHRFAM7A, CHRNA7, CHRNA7-DR1, D-10, CHRNA7-FAM7A fusion protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
46kDa
Observed MW
46kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CHRFAM7A (NP_647536.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CHRFAM7A antibody (144-07844) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CHRFAM7A antibody (144-07844) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CHRFAM7A antibody (144-07844) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CHRFAM7A antibody (144-07844) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CHRFAM7A antibody (144-07844) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CHRFAM7A antibody (144-07844) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CHRFAM7A antibody (144-07844) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.