Anti-CHRM5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P08912
Gene Id	1133
Gene Symbols	CHRM5
Protein Name / Synonyms	CHRM5, HM5, cholinergic receptor muscarinic 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

60kDa

Observed MW

68kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHRM5 antibody (144-05367) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.
Western blot - CHRM5 antibody (144-05367)

Immunohistochemistry of paraffin-embedded rat brain using CHRM5 antibody (144-05367) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHRM5 antibody (144-05367)

Immunofluorescence analysis of U2OS cells using CHRM5 antibody (144-05367). Blue: DAPI for nuclear staining.
Immunofluorescence - CHRM5 antibody (144-05367)

Expiration:

12 months from the date of shipment when stored properly.

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