RayBiotech

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ISO 13485:2016 cGMP

Anti-CHRNA5 Antibody

Catalog #: 144-01741

CHRNA5 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P30532
Gene Id
1138
Gene Symbols
CHRNA5
Protein Name / Synonyms
CHRNA5, LNCR2, cholinergic receptor nicotinic alpha 5 subunit
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
53kDa
Observed MW
50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-240 of human CHRNA5 (NP_000736.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHRNA5 antibody (144-01741) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CHRNA5 antibody (144-01741)

Expiration:
12 months from the date of shipment when stored properly.