Anti-CHRNB2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P17787
Gene Id	1141
Gene Symbols	CHRNB2
Protein Name / Synonyms	CHRNB2, EFNL3, nAChRB2, cholinergic receptor nicotinic beta 2 subunit
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

57kDa

Observed MW

50-52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 340-430 of human CHRNB2 (NP_000739.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHRNB2 antibody (144-09808) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CHRNB2 Polyclonal Antibody (144-09808)

Immunohistochemistry of paraffin-embedded mouse brain using CHRNB2 antibody (144-09808) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHRNB2 Polyclonal Antibody (144-09808)

Expiration:

12 months from the date of shipment when stored properly.

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