RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-CIAO1 Antibody

Catalog #: 144-07355

CIAO1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O76071
Gene Id
9391
Gene Symbols
CIAO1
Protein Name / Synonyms
CIAO1, CIA1, WDR39, cytosolic iron-sulfur assembly component 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
37kDa
Observed MW
38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human CIAO1 (NP_004795.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CIAO1 antibody (144-07355) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - CIAO1 antibody (144-07355)

Immunohistochemistry of paraffin-embedded rat brain using CIAO1 antibody (144-07355) at dilution of 1:100 (40x lens).
Immunohistochemistry - CIAO1 antibody (144-07355)

Immunohistochemistry of paraffin-embedded human kidney using CIAO1 antibody (144-07355) at dilution of 1:100 (40x lens).
Immunohistochemistry - CIAO1 antibody (144-07355)

Expiration:
12 months from the date of shipment when stored properly.