Anti-CIB1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q99828
Gene Id	10519
Gene Symbols	CIB1
Protein Name / Synonyms	CIB1, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, calcium and integrin binding 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	2.52 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. The encoded protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. The encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

21kDa/26kDa

Observed MW

20kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CIB1 (NP_006375.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CIB1 antibody (144-04430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - CIB1 antibody (144-04430)

Immunohistochemistry of paraffin-embedded human prostate using CIB1 antibody (144-04430) at dilution of 1:100 (40x lens).
Immunohistochemistry - CIB1 antibody (144-04430)

Expiration:

12 months from the date of shipment when stored properly.

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