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Anti-CKB Antibody
Catalog #: 130-10062
Rabbit Anti-Human CKB Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P12277 |
| Gene Id | 1152 |
| Gene Symbols | CKB CKBB |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from Human CKB. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa). |
| Antigen Catalog Number | 230-00039 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without preservative stabilizers, such as sodium azide. Reconstitute the antibody with sterile water. The final concentration is 1 mg/ml. Avoid repeated freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Creatine phosphokinase, also known as creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In cells, the "cytosolic" CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type)
Specificity
The rabbit anti-human CKB antibody specifically detects the target derived from human recombinant protein as well as cell lysates of 293T and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (suggesting with sterile PBS) can be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored at 4°C for 1 month. Store at -20°C, if not intended for use within a month. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Mouse Anti-Human Creatine Kinase BB(cat#DS-MB-01067)RayBio® Human Chemokine Antibody Array 1 (cat#AAH-CHE-1-8)
References
- Bürklen TS, et al.. (2007) Brain-type creatine kinase BB-CK interacts with the Golgi Matrix Protein GM130 in early prophase. Mol Cell Biochem. 297(1-2):53-64.
- Wallimann T, et al.. (1994) Creatine kinase in non-muscle tissues and cells. Mol Cell Biochem. 133-134:193-220.
Expiration:
12 months from the date of shipment when stored properly.