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Anti-CKB Antibody, Biotinylated
Catalog #: 130-10062B-50
Rabbit anti-CKB Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P12277 |
| Gene Id | 1152 |
| Gene Symbols | CKB CKBB |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from Human CKB. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Creatine phosphokinase, also known as creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In cells, the "cytosolic" CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type)
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human CKB antibody specifically detects the target derived from human recombinant protein as well as cell lysates of 293T and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Related Products
Mouse Anti-Human Creatine Kinase BB(cat#DS-MB-01067)RayBio® Human Chemokine Antibody Array 1 (cat#AAH-CHE-1-8)
References
- B¼rklen TS, et al.. (2007) Brain-type creatine kinase BB-CK interacts with the Golgi Matrix Protein GM130 in early prophase. Mol Cell Biochem. 297(1-2):53-64.
- Wallimann T, et al.. (1994) Creatine kinase in non-muscle tissues and cells. Mol Cell Biochem. 133-134:193-220.
Expiration:
12 months from the date of shipment when stored properly.