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Anti-Clatherin Light Chain Antibody (LCA/LCB)
Catalog #: 188-11072
Mouse anti-Human Clatherin Light Chain Antibody (LCA/LCB)
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P09496|P09497 |
| Gene Id | 1211|1212 |
| Gene Symbols | CLTA,CLTB |
| Protein Name / Synonyms | Clathrin light chain A (Lca), Clathrin light chain B (Lcb) |
| Immunogen | A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody. |
| Isotype | Kappa, IgG2b |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 min at RT |
| Domain / Region / Terminus | Cytoplasmic |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Storage
Store the Clathrin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.