Anti-CLIC1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O00299
Gene Id	1192
Gene Symbols	CLIC1
Protein Name / Synonyms	CLIC1, CL1C1, G6, NCC27, chloride intracellular channel 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

26kDa

Observed MW

27kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-241 of human CLIC1 (NP_001279.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CLIC1 antibody (144-60445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - CLIC1 Polyclonal Antibody (144-60445)

Immunofluorescence analysis of MCF7 cells using CLIC1 antibody (144-60445). Blue: DAPI for nuclear staining.
Immunofluorescence - CLIC1 Polyclonal Antibody (144-60445)

Expiration:

12 months from the date of shipment when stored properly.

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