Anti-CLIC4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y696
Gene Id	25932
Gene Symbols	CLIC4
Protein Name / Synonyms	CLIC4, CLIC4L, H1, MTCLIC, huH1, p64H1, chloride intracellular channel 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

28kDa

Observed MW

29kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CLIC4 antibody (144-07088) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 2s.
Western blot - CLIC4 Polyclonal Antibody (144-07088)

Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody (144-07088) at dilution of 1:100 (40x lens).
Immunohistochemistry - CLIC4 Polyclonal Antibody (144-07088)

Immunofluorescence analysis of A-549 cells using CLIC4 antibody (144-07088). Blue: DAPI for nuclear staining.
Immunofluorescence - CLIC4 Polyclonal Antibody (144-07088)

Expiration:

12 months from the date of shipment when stored properly.

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