Anti-CLTC Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q00610
Gene Id	1213
Gene Symbols	CLTC
Protein Name / Synonyms	CLTC, CHC, CHC17, CLH-17, CLTCL2, Hc, clathrin heavy chain
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

187kDa/191kDa

Observed MW

191kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1451-1675 of human CLTC (NP_004850.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CLTC antibody (144-012423) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - CLTC Polyclonal Antibody (144-012423)

Immunofluorescence analysis of HeLa cells using CLTC antibody (144-012423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - CLTC Polyclonal Antibody (144-012423)

Expiration:

12 months from the date of shipment when stored properly.

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