3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Coagulation factor III (F3) Antibody
Catalog #: 130-10150
Rabbit Anti-Coagulation factor III (F3) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_001984 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein F3.The membrane blot was probed with anti- F3 primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein F3 from E.coli cell (19kDa). |
| Antigen Catalog Number | 230-00221 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Coagulation factor III (F3), which is a cell surface glycoprotein, enables cells to initiate the blood coagulation cascades. F3 is the cell surface receptor for the serine protease factor VIIa, which functions as the high-affinity receptor.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat F3 has not yet been tested.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Recombinant Human Tissue Factor / F3 (Cat: 228-11517-1) ; Human L-507 Array, Glass Slide (2) (Cat: AAH-BLG-1-2)
References
- Raj S. Kasthuri, et al. Tissue Factor and Tissue Factor Pathway Inhibitor as Key Regulators of Global Hemostasis: Measurement of Their Levels in Coagulation Assays. Semin Thromb Hemost. 2010 October; 36(7): 764771.
- Saulius Butenas,et al. Posttranslational modifications and activity of natural and recombinant tissue factor. Thromb Res. 2010 April; 125S1: S26S28.
Expiration:
12 months from the date of shipment when stored properly.