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Anti-COL3A1 Antibody
Catalog #: 144-03795
COL3A1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P02461 |
| Gene Id | 1281 |
| Gene Symbols | COL3A1 |
| Protein Name / Synonyms | COL3A1, EDS4A, collagen alpha-1(III) chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
111kDa/138kDa
Observed MW
170kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1217-1466 of human COL3A1 (NP_000081.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using COL3A1 antibody (144-03795) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.