RayBiotech

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Anti-COL4A2 Antibody

Catalog #: 144-07657

COL4A2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P08572
Gene Id
1284
Gene Symbols
COL4A2
Protein Name / Synonyms
COL4A2, ICH, POREN2, collagen alpha-2(IV) chain
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaAngiogenesis
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
167kDa
Observed MW
200kDa

Immunogen

A synthetic peptide of human COL4A2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using COL4A2 antibody (144-07657) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - COL4A2 antibody (144-07657)

Expiration:
12 months from the date of shipment when stored properly.