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Anti-COL4A3BP Antibody

Catalog #: 144-61023

COL4A3BP Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9Y5P4
Gene Id
10087
Gene Symbols
COL4A3BP
Protein Name / Synonyms
COL4A3BP, CERT, CERTL, GPBP, MRD34, STARD11, collagen type IV alpha-3-binding protein
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
68kDa/70kDa/83kDa
Observed MW
71kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 369-598 of human COL4A3BP (NP_112729.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using COL4A3BP antibody (144-015376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - COL4A3BP antibody (144-015376)

Expiration:
12 months from the date of shipment when stored properly.