Anti-COMP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P49747
Gene Id	1311
Gene Symbols	COMP
Protein Name / Synonyms	COMP, EDM1, EPD1, MED, PSACH, THBS5, TSP5, cartilage oligomeric matrix protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	2.75 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW

77kDa/82kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using COMP antibody (144-05812) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - COMP Polyclonal Antibody (144-05812)

Immunofluorescence analysis of U2OS cells using COMP antibody (144-05812). Blue: DAPI for nuclear staining.
Immunofluorescence - COMP Polyclonal Antibody (144-05812)

Expiration:

12 months from the date of shipment when stored properly.

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