3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-COPB2 Antibody
Catalog #: 144-07036
COPB2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35606 |
| Gene Id | 9276 |
| Gene Symbols | COPB2 |
| Protein Name / Synonyms | COPB2, beta'-COP, coatomer subunit beta' |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 0.9 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
99kDa/102kDa
Observed MW
102kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using COPB2 antibody (144-07036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using COPB2 antibody (144-07036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.