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Anti-CORO1A Antibody
Catalog #: 144-09300
CORO1A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P31146 |
| Gene Id | 11151 |
| Gene Symbols | CORO1A |
| Protein Name / Synonyms | CORO1A, CLABP, CLIPINA, HCORO1, IMD8, TACO, p57, coronin-1A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
51kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 362-461 of human CORO1A (NP_001180262.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CORO1A antibody (144-09300) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunofluorescence analysis of rat brain using CORO1A antibody (144-09300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat heart using CORO1A antibody (144-09300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.