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Anti-COX7A2 Antibody
Catalog #: 144-08406
COX7A2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P14406 |
| Gene Id | 1347 |
| Gene Symbols | COX7A2 |
| Protein Name / Synonyms | COX7A2, COX7AL, COX7AL1, COXVIIAL, COXVIIa-L, VIIAL, cytochrome c oxidase subunit 7A2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
9kDa
Observed MW
11kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 33-115 of human COX7A2 (NP_001856.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using COX7A2 antibody (144-08406) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Expiration:
12 months from the date of shipment when stored properly.