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Anti-CPOX Antibody
Catalog #: 144-03807
CPOX Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P36551 |
| Gene Id | 1371 |
| Gene Symbols | CPOX |
| Protein Name / Synonyms | CPOX, CPO, CPX, HCP, coproporphyrinogen oxidase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
30kDa/50kDa
Observed MW
36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 111-454 of human CPOX (NP_000088.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CPOX antibody (144-03807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using CPOX antibody (144-03807) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CPOX antibody (144-03807) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CPOX antibody (144-03807) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CPOX antibody (144-03807) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.