Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16630 |
| Gene Id | 11052 |
| Gene Symbols | CPSF6 |
| Protein Name / Synonyms | CPSF6, CFIM, CFIM68, HPBRII-4, HPBRII-7, CFIM72, cleavage and polyadenylation specific factor 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.
Dilutions
WB 1:1000 - 1:3000
Format
Calculated MW
52kDa/59kDa/63kDa
Observed MW
70kDa
Immunogen
Recombinant protein of human CPSF6
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CPSF6 antibody (144-05963) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.
