Anti-CRABP2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P29373
Gene Id	1382
Gene Symbols	CRABP2
Protein Name / Synonyms	CRABP2, CRABP-II, RBP6, cellular retinoic acid-binding protein 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100

Format

Calculated MW

15kDa

Observed MW

15kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-94 of human CRABP2 (NP_001869.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CRABP2 antibody (144-06119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Western blot - CRABP2 Polyclonal Antibody (144-06119)

Immunohistochemistry of paraffin-embedded rat brain using CRABP2 antibody (144-06119) at dilution of 1:100 (40x lens).
Immunohistochemistry - CRABP2 Polyclonal Antibody (144-06119)

Immunofluorescence analysis of U2OS cells using CRABP2 antibody (144-06119). Blue: DAPI for nuclear staining.
Immunofluorescence - CRABP2 Polyclonal Antibody (144-06119)

Expiration:

12 months from the date of shipment when stored properly.

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