Anti-CREB1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P16220
Gene Id	1385
Gene Symbols	CREB1
Protein Name / Synonyms	CREB1 (Center)
Clonality	Polyclonal
Preparation	This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1.
Concentration (lot specific)	0.252 mg/ml
Recommended Applications	FC, Western Blotting, IF
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

CREB1; Cyclic AMP-responsive element-binding protein 1

NCBI Accession #

NP_004370.1;NP_604391.1

Other Accession #

P15337; Q01147; P27925; NP_004370.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

36688

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; FC: 1:10-50

	Fluorescent image of A549 cell stained with CREB1 Antibody (Center)(Cat# 102-11218).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREB1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).CREB1 immunoreactivity is localized to Nucleus significantly.
	CREB1 Antibody (Center) (Cat# 102-11218) western blot analysis in HT29 cell line lysates (35ug/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow).
	CREB1 Antibody (Center) (Cat# 102-11218) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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