Anti-CRKL Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P46109
Gene Id	1399
Gene Symbols	CRKL
Protein Name / Synonyms	CRKL, crk-like protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

33kDa

Observed MW

37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CRKL Antibody (144-11735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] CRKL Polyclonal Antibody (144-11735)

Western blot analysis of extracts from normal (control) and CRKL knockout (KO) HeLa cells, using CRKL antibody (144-11735) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - [KO Validated] CRKL Polyclonal Antibody (144-11735)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products