Anti-CSNK1E Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P49674
Gene Id	1454
Gene Symbols	CSNK1E
Protein Name / Synonyms	CSNK1E, CKIepsilon, HCKIE, casein kinase 1 epsilon
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

47kDa

Observed MW

47kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CSNK1E antibody (144-01796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (144-01796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (144-01796) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (144-01796) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (144-01796) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (144-01796) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Immunofluorescence analysis of HeLa cells using CSNK1E antibody (144-01796). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)

Expiration:

12 months from the date of shipment when stored properly.

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