RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-CST1 Antibody

Catalog #: 144-06570

CST1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P01037
Gene Id
1469
Gene Symbols
CST1
Protein Name / Synonyms
CST1, cystatin-SN
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
16kDa
Observed MW
16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-141 of human CST1 (NP_001889.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CST1 Antibody (144-06570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - CST1 Antibody (144-06570)

Immunohistochemistry of paraffin-embedded rat brain using CST1 antibody (144-06570) at dilution of 1:100 (40x lens).
Immunohistochemistry - CST1 antibody (144-06570)

Immunohistochemistry of paraffin-embedded human prostate using CST1 antibody (144-06570) at dilution of 1:100 (40x lens).
Immunohistochemistry - CST1 antibody (144-06570)

Immunohistochemistry of paraffin-embedded mouse stomach using CST1 antibody (144-06570) at dilution of 1:100 (40x lens).
Immunohistochemistry - CST1 antibody (144-06570)

Expiration:
12 months from the date of shipment when stored properly.