RayBiotech

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ISO 13485:2016 cGMP

Anti-CSTF1 Antibody

Catalog #: 144-05915

CSTF1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q05048
Gene Id
1477
Gene Symbols
CSTF1
Protein Name / Synonyms
CSTF1, CstF-50, CstFp50, cleavage stimulation factor subunit 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
48kDa
Observed MW
50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CSTF1 (NP_001315.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CSTF1 antibody (144-05915) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - CSTF1 antibody (144-05915)

Expiration:
12 months from the date of shipment when stored properly.