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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49711 |
| Gene Id | 10664 |
| Gene Symbols | CTCF |
| Protein Name / Synonyms | CTCF, MRD21, CCCTC-binding factor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP, ChIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
ChIP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
ChIP 1:50 - 1:200
Format
Calculated MW
45kDa/82kDa
Observed MW
135kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CTCF antibody (144-60257) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody (144-60257) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody (144-60257) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CTCF antibody (144-60257) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CTCF antibody (144-60257) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (144-60257). Western blot was performed from the immunoprecipitate using CTCF antibody (144-60257) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibody (144-60257) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.