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Anti-CTNNB1 Antibody
Catalog #: 144-11512
CTNNB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35222 |
| Gene Id | 1499 |
| Gene Symbols | CTNNB1 |
| Protein Name / Synonyms | CTNNB1, CTNNB, MRD19, armadillo, catenin beta-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
9kDa/85kDa
Observed MW
105kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CTNNB1 (NP_001895.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CTNNB1 antibody (144-011512) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot analysis of extracts from normal (control) and CTNNB1 knockout (KO) HeLa cells, using CTNNB1 antibody (144-011512) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using CTNNB1 antibody (144-011512) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using CTNNB1 antibody (144-011512) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of U-87MG cells, using 3 ug CTNNB1 antibody (144-011512). Western blot was performed from the immunoprecipitate using CTNNB1 antibody (144-011512) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.