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Anti-CTNNB1 (C-term) Antibody
Catalog #: 102-20300
Mouse Anti-CTNNB1 (C-term) Antibody, 200 µl
Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Mouse |
| Accession Number | P35222|Q0VCX4|B6V8E6|Q02248|Q9WU82 |
| Gene Id | 1499 |
| Gene Symbols | CTNNB1 |
| Protein Name / Synonyms | Catenin beta-1, Beta-catenin, CTNNB1, CTNNB |
| Clonality | Monoclonal |
| Clone | 691CT11.2.1 |
| Isotype | IgG1 |
| Recommended Applications | IF, Western Blotting |
| Research Area | Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
Other Information
Antigen Type
Synthesized Peptide
Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Calculated Molecular Weight (Da)
85497
Antigen Source
Human
Images
IF (1:25)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling CTNNB1 with 102-20300 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.