RayBiotech

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ISO 13485:2016 cGMP

Anti-CTSH Antibody

Catalog #: 144-05368

CTSH Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P09668
Gene Id
1512
Gene Symbols
CTSH
Protein Name / Synonyms
CTSH, ACC-4, ACC-5, ACC4, ACC5, CPSB, cathepsin H
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
37kDa
Observed MW
32kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of human CTSH (NP_004381.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CTSH antibody (144-05368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CTSH antibody (144-05368)

Expiration:
12 months from the date of shipment when stored properly.