Anti-CXXC1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9P0U4
Gene Id	30827
Gene Symbols	CXXC1
Protein Name / Synonyms	CXXC1, 2410002I16Rik, 5830420C16Rik, CFP1, CGBP, HsT2645, PCCX1, PHF18, SPP1, ZCGPC1, hCGBP, CXXC finger protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that functions as a transcriptional activator that binds specifically to non-methylated CpG motifs through its CXXC domain. The protein is a component of the SETD1 complex, regulates gene expression and is essential for vertebrate development.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

75kDa/76kDa

Observed MW

75kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human CXXC1 (NP_001095124.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CXXC1 antibody (144-05814) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - CXXC1 antibody (144-05814)

Immunohistochemistry of paraffin-embedded mouse lung using CXXC1 antibody (144-05814) at dilution of 1:200 (40x lens).
Immunohistochemistry - CXXC1 antibody (144-05814)

Immunofluorescence analysis of A549 cells using CXXC1 antibody (144-05814). Blue: DAPI for nuclear staining.
Immunofluorescence - CXXC1 antibody (144-05814)

Expiration:

12 months from the date of shipment when stored properly.

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